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Tag: Flow Cytometry

The CTEGD Cytometry Shared Resource Lab is growing!

Donna Huber on December 7, 2022

CTEGD’s Cytometry Shared Resource Laboratory recently added a Malvern Panalytical NanoSight NS300 to their suite of cytometry instruments. This was a donation from the laboratory of Dr. Stephen Hajduk.

The NanoSight allows rapid analysis of the size distribution and concentration of all types of nanoparticles from 0.01 – 1 µm in diameter.  It is important to monitor and control the isolation and purification of extracellular vesicles (EVs) while conducting research into their function. NanoSight NTA (Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion to provide quick and easy characterization of both the size and concentration of vesicles in aqueous buffers, giving confidence in the quality of the sample used in any downstream experiments.

Researchers across the UGA campus are investigating the role of EVs in T. brucei and N. fowleri infections, neural stem cell extracellular vesicle uptake and neutrophil uptake of exosomes isolated from cystic fibrosis sputum.

The NanoSight is the second instrument CSRL added in 2022 (see the Cytek Aurora Spectral Cytometer announcement) and increases the number of cytometry instruments available for CTEGD and the UGA research community use to 11.

The CSRL continues to provide access to state-of-the-art cytometry analyzers and sorters to researchers at the University of Georgia and across the scientific community. In addition to the instruments, the facility also provides expert advice and consultation for the design and analysis of flow experiments.

Cytometry Shared Resource Lab expands capabilities with new instrument

Donna Huber on January 20, 2022

Julie Nelson
Julie Nelson, CSRL manager, is available to train users on the newly acquire CYTEK Aurora flow cytometer. (photo credit: Donna Huber)

With the generous financial support of The Office of Research, Cytometry Shared Resource Lab, Center for Tropical and Emerging Global Diseases, Department of Infectious Diseases, and UGA faculty members Rick Tarleton, Mark Tompkins, Chet Joyner, and Sam Kurup, the CTEGD Cytometry Shared Resource Laboratory (CSRL) recently added the Cytek Aurora Spectral Cytometer to its facility. This new instrument provides a high level of flexibility to the researcher and complements the other instruments available at the facility.

“It’s great to be able to bring this exciting new resource to campus in support of the incredible research UGA members are conducting,” said Julie Nelson, CSRL manager.

Flow cytometry is a technique for measuring characteristics of cells or particles using laser excitation and innate fluorescence emission or emission from dyes added to identify cells or particles and their function in experimental biology.  In the past 30 years, this technology has revolutionized many areas of cell biology research including the study of viruses, bacteria, infectious diseases, and cancer.  This technology has also proven useful in genetic studies of plants.

The Aurora Spectral Cytometer delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity. With 64 emission detectors and 5 lasers for excitation, the Aurora can resolve almost any fluorescent marker currently on the market. It can also evaluate dyes under development that can be excited by one or more of the 5 lasers available.

“This cytometer expands our capabilities from a limit of 24 parameters to 64 making it ideal for high dimensional immunophenotyping,” said Nelson. “But because of its unique spectral emission detection, it is also the best instrument for looking at highly autofluorescent cells, such as macrophages and liver cells, regardless of how many markers are needed for the assay.”

For more than 20 years, the CSRL has provided access to state-of-the-art flow cytometry analyzers to researchers at the University of Georgia and across the scientific community. In addition to the instruments, the facility also provides expert advice and consultation for the design and analysis of flow experiments.

The CSRL is hosting a free webinar on the CYTEK Aurora on Friday, January 28 at 1:00 pm with Christopher Fleming, Ph.D. To register: https://register.gotowebinar.com/register/2294357931236562192

webinar flyer

Celebrate the 10th Anniversary of SEFCIG

Donna Huber on February 7, 2019

SECFIG annual meeting

Celebrate the 10th anniversary of the Southeast Flow Cytometry Interest Group (SEFCIG) with 4 days of educational training and scientific talks. SEFCIG was founded by Julie Nelson, the director of UGA’s CTEGD Cytometry Shared Resource Laboratory. The 10th Annual Meeting is being hosted on the University of Georgia campus in Athens, GA.

10th Annual Meeting March 5 – 8, 2019

March 5 – 6         ExCyte – Flow Cytometry Boot Camp

Register here: https://excyteatuga.eventbrite.com/

Special discounts are available to the UGA community and those attending SEFCIG, please email Tim Bushnell (timbushnell@expertcytometry.com) for details.

March 7 – TechnoFlow

Location: Paul D. Coverdell Building Room S175

11:00 – 12:00

Forensic Flow – Join us to test your skills at detecting bad flow data
Jodi Kroeger, Moffitt Cancer Institute

12:00 – 1:00

 Luncheon – Coverdell Rotunda

1:00 – 1:45

Multi-Dimensional Functional Profiling of Human Rhinovirus and Allergen- specific T-Cells By Means Of Spectral Flow Cytometry
Liesbeth Paul and Joanne Lannigan, University of Virginia

1:45 – 2:30

 Imaging and Spectral Cytometry go Viral!
Joanne Lannigan, University of Virginia

2:30 – 3:15

High Dimensional Mass Cytometry Data Analysis
Deon Bryant, Emory University

3:15 – 4:00

Next Generation Cell Sorting: New Technologies and Strategies
Joe Trotter, Becton Dickinson

6:00 – 9:00

Opening Reception – Pecan Tree Galleria

March 8 – General Session

Location: Masters Hall, Georgia Center for Continuing Education

Flow in the South

9:00 – 9:10

Welcome and Introductions

9:10 – 9:55

Canine Breast Cancer Immunotherapy as a Model of Human Disease
Curtis Bird, Auburn University

9:55 – 10:40

Single cell analyses of human B cell responses: Lessons from infectious disease and autoimmunity
Jens Wrammert, Emory University

10:40 – 11:25

Using Flow Cytometry to Catch Parasites Sleeping
Dennis Kyle, University of Georgia

11:25 – 12:00

Exhibits

Vendor Break Out Session with Box Lunches

12:00 – 2:45

Vendor Talks and Exhibits

Flow in the World

2:45 – 3:30

Extracellular Vesicle-Biome Analysis by Nanoscale High Resolution Flow Cytometry
Terry Morgan, Oregon School of Health

3:30 – 4:15

Optimizing and validating FC-based EV measurements
John Nolan, Scintillon Institute

4:15 – 5:00

Developing Flow Cytometry Assays to Support Clinical Trials
Jake Jacobberger, Case Western

Register and make hotel reservations at www.sefcig.org