CTEGD’s Cytometry Shared Resource Laboratory recently added a Malvern Panalytical NanoSight NS300 to their suite of cytometry instruments. This was a donation from the laboratory of Dr. Stephen Hajduk.
The NanoSight allows rapid analysis of the size distribution and concentration of all types of nanoparticles from 0.01 – 1 µm in diameter. It is important to monitor and control the isolation and purification of extracellular vesicles (EVs) while conducting research into their function. NanoSight NTA (Nanoparticle Tracking Analysis (NTA) utilizes the properties of both light scattering and Brownian motion to provide quick and easy characterization of both the size and concentration of vesicles in aqueous buffers, giving confidence in the quality of the sample used in any downstream experiments.
Researchers across the UGA campus are investigating the role of EVs in T. brucei and N. fowleri infections, neural stem cell extracellular vesicle uptake and neutrophil uptake of exosomes isolated from cystic fibrosis sputum.
The NanoSight is the second instrument CSRL added in 2022 (see the Cytek Aurora Spectral Cytometer announcement) and increases the number of cytometry instruments available for CTEGD and the UGA research community use to 11.
The CSRL continues to provide access to state-of-the-art cytometry analyzers and sorters to researchers at the University of Georgia and across the scientific community. In addition to the instruments, the facility also provides expert advice and consultation for the design and analysis of flow experiments.