Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors

Category: CTEGD Blog

Trainee Spotlight: Molly Bunkofse

trainee Molly Bunkofse

NIH T32 trainee Molly Bunkofse, a Ph.D. student in Rick Tarleton‘s laboratory, is originally from Illinois and I obtained my BA in Biology from a small, liberal arts college called Augustana, which is located in Rock Island, IL.

Molly’s research focus

Molly’s project focuses on the host CD8+ T cell response that is generated against flagellar proteins from the parasite Trypanosoma cruzi and exploring how these responses might be enhanced.

“I chose this research focus because I have always been interested in the host immune response to pathogens and especially pathogens that are able to escape the immune response and persist, such is the case in T. cruzi infection.”

Trainee capstone experience

Each T32 trainee is provided with the opportunity to complete a capstone experience at the end of their fellowship. This experience allows for an extended visit to a collaborator’s laboratory or travel to a scientific meeting where they present their research and interact with colleagues.

“For my capstone experience, I’d like to work in South America where Chagas disease is endemic, perhaps with one of our collaborators that works with human patients infected with T. cruzi.”

T32 fellowship helps trainees achieve their goals

“I think that the T32 fellowship will provide me with new opportunities to develop my research and skills as a scientist. The experiences and training will enable me to become a well-rounded scientist that can think critically and logically approach a question/problem.”

Molly hopes to continue her research in a government laboratory after graduation.

Trainee Spotlight: Ruby Harrison

trainee Ruby Harrison

NIH T32 trainee Ruby Harrison is a co-advised by Drs. Michael Strand and Mark Brown in the UGA Department of Entomology. She received a Bachelor’s of Science in Entomology from the University of Wisconsin-Madison in 2012 and lived in Madison an additional two years working with mosquitoes as a research assistant. Before coming to UGA to begin my doctoral studies, she spent a year in Gabon, Africa, working as a tropical ecology field technician.

Ruby’s research focus

Ruby studies mosquito-microbiome interactions. Currently, she is investigating the influence of the gut microbiome on mosquito reproductive processes. She also plans to begin exploring the role of the mosquito microbiome in deterring pathogen infection in the very near future.

“I chose this research focus because I was inspired by the research of a former graduate student of Dr. Strand’s, Dr. Kerri Coon. Kerri pioneered fascinating work on the influence of the microbiota on development in mosquitoes in the immature (larval) stage,” said Ruby. “I saw an opportunity to extend her work, to observe if the same bacterial signal essential to larval development is recapitulated in any way in the adult stage.”

More broadly, she sees insect-microbe interactions as a promising field which may offer new solutions for mosquito population control and reduction of pathogen transmission.

NIH T32 Fellowship helps trainees achieve their goals

Ultimately, Ruby hopes to build a career as a vector biologist. For the capstone experience provided by the NIH T32 Training Grant, she is interested in returning to francophone West or Central Africa to work with mosquitoes in the field.

“I am truly grateful to receive the T32 pre-doctoral training fellowship, which presents me the opportunity to interact more closely with the CTEGD, opens doors for possible collaboration, and will help me to pursue my research goals,” said Ruby.

An ancient bacterial protein complex in human malaria parasites is essential for parasite growth

Vasant Muralidharan and Anat Florentin

Several species of Plasmodium parasites cause malaria in humans and results in nearly 450,000 deaths annually. The deadliest of these species is Plasmodium falciparum. Unfortunately, it is also drug resistance to many of the currently available treatments. Vasant Muralidharan, assistant professor in the department of cellular biology, and his research group at The Center for Tropical and Emerging Global Diseases at The University of Georgia reported on an essential protein in hopes of identifying new drug targets.

Plasmodium parasites contain an organelle known as the apicoplast that evolved via the endosymbiosis of a red alga. The apicoplast produces several essential metabolites required for parasite growth and survival. Therefore, drugs that target the apicoplast are clinically effective. However, there is still not a lot known about this organelle. Understanding the function, structure, and biogenesis of the apicoplast provides a gold mine of antimalarial drug targets.

The role of Clp proteins in Plasmodium apicoplast

Clp (Caseinolytic Proteases) are conserved prokaryotic proteins that serve a wide variety of biological functions in bacteria, the evolutionary ancestors of the apicoplast. Several Clp proteins have been reported to localize in the apicoplast of the parasite but their biological functions were unknown.

The research team used different genetic tools to conditionally inhibit the function of various apicoplast-Clp proteins. “It is similar to understanding the role of a single card in holding up a house of cards by removing it from the structure,” said Muralidharan.

Their data show that the Clp chaperone PfClpC is essential for parasite viability and that its inhibition resulted in morphological defects, and loss of the apicoplast. They also revealed that the chaperone activity is required to stabilize a Clp Protease, PfClpP, suggesting that, similar to bacteria and plants chloroplasts, these two proteins form a proteolytic complex. These data may be relevant to the function of bacterial and plant Clp complexes. “Our findings shed light on the biological roles of the apicoplast Clp Proteins and their involvement in apicoplast replication,” said Dr. Anat Florentin, lead author on the study.

Significance of the findings

The role that bacterial Clp proteins play in cell division, stress response and ability to cause disease have placed them at the center of several drug discovery programs. The new understanding of Clp proteins in Plasmodium provides an avenue for drug development in malaria in which highly active antibacterial compounds can be repurposed as effective anti-malarial agents.

 

An online version of this study is available: A. Florentin,  D.W. Cobb, J.D. Fishburn, M. J. Cipriano, P.S. Kim, M.A. Fierro, B. Striepen, V. Muralidharan. 2017. PfClpC is an essential Clp chaperone required for plastid integrity and Clp protease stability in Plasmodium falciparum. Cell Reports 21, 1 – 11. http://dx.doi.org/10.1016/j.celrep.2017.10.081

Assistant professor focuses research on environmental drivers of disease

Courtney Murdock was well on her way to becoming a veterinarian; as a pre-vet student at the University of Michigan she majored in biology and volunteered at a small animal hospital. But then she spent the summer before her senior year working at the university’s biological field station.

“I learned about general ecology and field mammalogy, and by the end of that summer, I knew I wanted to study ecology in graduate school,” she said.

For her doctoral work, also at the University of Michigan, she researched avian blood parasites, becoming interested in the ecology of disease transmission. That led her to Penn State University where she spent five years as a postdoctoral research scholar studying the ecology of disease vectors, the living organisms that carry and transmit diseases.

Now Murdock has come full circle. In 2014 she joined the faculty at UGA as an assistant professor with a joint appointment in the Odum School of Ecology and the College of Veterinary Medicine infectious diseases department.

Murdock’s research program is focused on the environmental drivers of disease transmission and the ecology of vector-borne diseases. She is particularly interested in two types of diseases that are transmitted by mosquitoes: human malaria and arboviruses, which include dengue and Zika.

She and her group combine lab and field experiments with computer modeling to generate predictions about disease spread and to evaluate disease control strategies in the context of changing environmental conditions.

As the recent outbreak of Zika demonstrated, the stakes are high.

“Often mosquito interventions are the only way to mitigate these diseases,” Murdock said, which is why understanding how variables like temperature, rainfall and land use affect mosquitoes’ capacity for transmitting disease is so important.

People want to learn about these issues. They want to be engaged. — Courtney Murdock

Murdock’s research interests inform her teaching, which includes an upper-level undergraduate/graduate course on the population biology of infectious diseases. A participating faculty member in the Infectious Disease Ecology Across Scales interdisciplinary doctoral training program, she also enjoys teaching general ecology for undergraduates.

“It broadens your thinking,” she said. “And working with students with different skill sets and backgrounds has helped me become a better mentor.”

Murdock’s teaching goes beyond the classroom. She is currently developing an Athens-based outreach project to inform people about the risks—and how to mitigate them—of living with mosquitoes, and she recently taught a course on Zika for the UGA Osher Lifelong Learning Institute.

“People want to learn about these issues,” she said. “They want to be engaged.”

The kind of diversity she values in her students is something Murdock also appreciates about her faculty colleagues.

“The appeal of UGA was the joint position with the College of Veterinary Medicine and the Odum School, and having access to so many renowned colleagues with different expertise than mine,” she said.

Murdock cited the Faculty of Infectious Diseases, the Center for Tropical and Emerging Global Diseases and the Center for the Ecology of Infectious Diseases as important resources that make UGA an ideal place for her to work.

“If you’re curious, ecology is a good field,” said Murdock. “It’s theory driven: you make hypotheses and design experiments to test. There are a lot of major problems that can benefit from an ecological perspective and integration from other fields, like antibiotic resistance and urbanization. This is a great time, and a great place, to be an ecologist.”

First published in Columns.

New UGA Drug Discovery Core lab works to develop treatment of leading diseases

Drug Discovery Center team
Members of the Drug Discovery Core steering committee in the new DDC laboratory (from left to right): Shelley Hooks, interim director of the Center for Drug Discovery and associate professor of pharmaceutical and biomedical sciences; Scott Pegan, chair of the steering committee and associate professor of pharmaceutical and biomedical sciences; Belen Cassera, associate professor of biochemistry and molecular biology; Kojo Mensa-Wilmot, professor and head of UGA’s cellular biology department and director of the Chemical Biology Group; and Brian Cummings, director of the Interdisciplinary Toxicology Program and professor of pharmaceutical and biomedical sciences.

Athens, Ga. – The University of Georgia has created the Drug Discovery Core laboratory, a campus-wide collaborative facility designed to hasten the development of therapeutic drugs for a number of major diseases.

A survey distributed to UGA researchers in 2016 identified chemical screening and toxicity profiling as the most critical needs for enhancing drug discovery research at UGA, and the DDC will address many of those needs for faculty working in infectious disease, regenerative medicine, cancer biology and other human health-focused disciplines.

Phase one of the new lab will allow for the curation, management and distribution of chemical libraries containing more than 50,000 compounds. The lab also will enable researchers to rapidly screen these chemical libraries in miniaturized models of various diseases using robotics and high-throughput signal detection. Finally, the lab will provide opportunities to identify potential toxicity of the compounds and determine if their chemical properties will allow them to be successfully delivered to patients. Additional capabilities, including pharmacokinetic characterization, genotoxicity and assay design, are under development.

“The most immediate outcome of the DDC lab will be to generate preliminary data from pilot chemical screens, which is necessary to secure large drug discovery grants from the National Institutes of Health to fund more advanced drug discovery research,” said Shelley Hooks, interim director of the Center for Drug Discovery and associate professor of pharmaceutical and biomedical science. “The longer-term goals of the lab are to discover and develop new drug candidates and chemical probes, as well as enhance training of graduate students in biotechnology.”

Creation of the DDC was initiated by Hooks in collaboration with Brian Cummings, director of the Interdisciplinary Toxicology Program and professor in the pharmaceutical and biomedical sciences department, and Scott Pegan, chair of the DDC steering committee and associate professor of pharmaceutical and biomedical sciences.

Sponsoring campus organizations include the College of Pharmacy, the College of Veterinary Medicine, the Office of Research, the Center for Tropical and Emerging Diseases and the Department of Cellular Biology.

The laboratory is located in Room 224 of the Wilson Building in the College of Pharmacy. For more information on capability, resources and access to the libraries and screening instruments, contact Pegan (spegan@uga.edu) or see cdd.rx.uga.edu.

Writer: Mickey Y. Montevideo
Contact: Shelley B. Hooks

Patrick Lammie Awarded Donald Mackay Medal

Pat LammiePatrick Lammie, CTEGD member and adjunct faculty in the Department of Cellular Biology, was awarded the Donald Mackay Medal during the American Society for Tropical Medicine and Hygiene’s (ASTMH) annual meeting in Baltimore, MD.

The Donald Mackay Medal recognizes outstanding work in tropical health, especially relating to improvements in the health of rural or urban workers in the tropics. In a long-standing partnership with the Royal Society of Tropical Medicine and Hygiene (RSTMH), the Donald Mackay Medel is awarded in odd years by ASTMH and in even years by RSTMH.

CTEGD at ASTMH Annual Meeting

Many of CTEGD researchers and collaborators will be at the American Society for Tropical Medicine and Hygiene’s Annual meeting in Baltimore, MD next week. Below is a list of presentations where you can hear about the work we are doing.

November 5

Session Parasitology (ACMCIP) Pre-Meeting Course: Single Cell Biology for Parasitologists – 2:00 – 2:45 pm, Hilton Holiday Ballroom 4 (East Building, 2nd Floor)

Bioinformatics Approaches to Single Cell Parasitology

Presenter: Jessica Kissinger

November 6

Session 24 – Protozoa – 10:30 – 10:45 am, Convention Center Room 337/338

84A natural mouse model for cryptosporidiosis

Presenter: Adam Sateriale (Striepen Research Group)

Session 15 – What Kinds of Molecules are Needed to Control and Eradicate Malaria? – 10:55 – 11:15 am, Convention Center Ballroom I (Level 400)

TCP3: Strategies for identifying novel anti-relapse agents

Presenter: Dennis Kyle, director of CTEGD

Session 28 – Poster Session A – 12:00 – 1:45 pm, Convention Center – Hall F and G (level 100)

LB-5052 – Drug Resistant Phenotypes for Artemisinins in GFP Expression Plasmodium falciparum From SE Asia

Presenter: Vivian Padin-Irizarry (Kyle Research Group)

LB-5033 – PfGRP170 is an essential ER protein in the human malaria parasite, Plasmodium falciparum

Presenter: Heather Bishop (Muralidharan Research Group)

November 7

Session 84 – Kinetoplastida: Molecular Biology and Immunology – 10:15 – 10:30 am, Convention Center Room 341/342

768 – Alterations in the IL27 pathway are correlated with the loss of Trypanosoma cruiz-specific T cells in patients with chronic Chagas Disease

Presenter: Maria Natale (collaborator, Tarleton Research Group)

Session 86 – Poster Session B – 12:00 -1:45 pm Convention Center – Hall F and G (level 100)

LB5207 – Investigating the role of PfHsp70x, the sole parasite exported Hsp70, in the display of antigens on the surface of the P. falciparum infected RBC

Presenter: David Cobb (Muralidharan Research Group)

877Resolving Temperature-driven Malaria Transmission Models

Presenter: Kerri Miazgowicz (Murdock Research Group)

1039 – EuPathDB: Powerful Data-Mining Tools for Exploring the Biology of Host-Pathogen Interactions

Presenter: Susanne Warrenfeltz (Kissinger Research Group)

Session 106 – Science is Real: Climate Change Impacts on Vector Borne-Diseases – 4:20 – 4:40 pm, Convention Center Ballroom IV (level 400)

Estimating vector-borne disease transmission in a variable environment

Presenter: Courtney Murdock

November 8

Session 145 – Poster Session C – 12:00 – 1:45 pm, Convention Center, Hall F and G (level 100)

LB-5362 – Global metabolic profiles differentiate acute and chronic malaria in rhesus macaques and humans

Presenter: Regina Joice Cordy (collaborator, Kissinger Research Group)

1672 – Multi-omic analysis of severity of infection in Macaca mulatta infected with Plasmodium cynomolgi

Presenter: Juan Gutierrez (collaborator, Kissinger Research Group)

LB-5397 – Enhancement of three closely-related species of Cryptosporidium genome annotation resources

Presenter: Rodrigo Baptista (Kissinger Research Group)

November 9

Session 192 – ACMCIP: Malaria and Protozoal Diseases – Biology and Pahtogenesis – 11:15 – 11:30 am, Convention Center Room 321/322/323 (level 300)

2013 – A natural mouse model for cryptosporidiosis

Presenter: Adam Sateriale (Striepen Research Group)

Session 188 – Malaria: Application of Innovative Technologies – 11:30 – 11:45 am, Convention Center Ballroom II (level 400)

1987 – Characterization of physiological signatures of Plasmodium infections in nonhuman primates using a continuous telemetry system

Presenter: Jessica Brady (collaborator, Kissinger Research Group)

Trainee Spotlight: Msano Mandalasi

trainee Msano Mandalasi
Msano Mandalasi, a post-doctoral trainee in Chris West‘s laboratory, is originally from Malawi, (located in southeastern Africa) and obtained her bachelor’s degree in Chemistry from the University of Malawi. After graduation, she worked briefly for the University of Malawi and then came to the US to obtain a Master’s degree in Chemistry. Later, she enrolled in a doctoral graduate program at the University of Maryland Eastern Shore where she graduated in 2012. She spent two years teaching undergraduate Chemistry before deciding to get back into research. She joined Dr. West’s group while he was at the University of Oklahoma and moved with the lab to the University of Georgia.

Msano’s research focus

The focus of Msano’s project is on the role of prolyl hydroxylation and glycosylation of E3 Ubiquitin ligase on Toxoplasma growth.

With a research background mostly in chemistry and biochemistry, her graduate research introduced her to some aspect of parasitology working on Schistosome glycobiology. However, she did not have a strong background in molecular biology prior to joining the West lab. This current project merges glycobiology and molecular biology and also extends some parasitology studies, thus giving her the opportunity to learn molecular biology and parasitology to complement her chemistry background. A combination of this expert knowledge will benefit her to address the research objectives on her Toxoplasma project.

Capstone experience

Each T32 trainee is provided with the opportunity to complete a capstone experience at the end of their fellowship. This experience allows for an extended visit to a collaborator’s laboratory or travel to a scientific meeting where they present their research and interact with colleagues. Msano plans to use her capstone experience to give oral presentations at scientific meetings, to publish some of the studies conducted within this time period, and interact with other trainees in the program.

T32 fellowship helps trainees achieve their goals

“Through the funding provided by the T32 Training Grant, I will be able to address research questions that should lead to launching my own area of research,” said Msano.

Msano hopes to run her own independent research program in academia one day.

 

Support trainees like Msano Mandalasi by giving to the Center for Tropical & Emerging Global Diseases

[button size=’large’ style=” text=’Give Online’ icon=” icon_color=’#b80d32′ link=’https://ctegd.uga.edu/give/’ target=’_self’ color=” hover_color=” border_color=” hover_border_color=” background_color=” hover_background_color=” font_style=” font_weight=” text_align=’center’ margin=”]